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KMID : 0620920000320010012
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Experimental & Molecular Medicine
2000 Volume.32 No. 1 p.12 ~ p.17
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Protective mechanism of glucose against alloxan-induced b bb b-cell damage: Pivotal role of ATP
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Jin Woo Park/Hye Won Rho
Ji Na Lee/Hyung Rho Kim/Byung Hyun Park/Jin Woo Park
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Abstract
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Glucose prevents the development of diabetes induced by alloxan. In the present study, the protective mechanism of glucose against alloxan-induced β-cell damage was investigated using HIT-T 15 cell, a Syrian hamster transformed β-cell line. Alloxan caused β-cell damages with DNA fragmentation, inhibition of glucose-stimulated insulin release, and decrease of cellular ATP level, but all of these β-cell damages by alloxan were prevented by the presence of 20 mM glucose. Oligomycin, a specific inhibitor of ATP synthase, completely abolished the protective effects of glucose against alloxan-induced cell damage. Furthermore, treatment of nuclei isolated from HIT-T15 cells with ATP significantly prevented the DNA fragmentation induced by Ca2+. The results indicate that ATP produced during glucose metabolism plays a pivotal role in the protection of glucose against alloxan-induced β-cell damage.
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KEYWORD
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glucose, ATP, alloxan, diabetes, DNA frag-mentation,
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